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Kevin McKernan posted the following comments about this blog post (twitter.com/Kevin_McKer…):
"Reads don't map to the margin of a reference because the given insert size you have selected in library construction won't exist. If you have 200bp inserts, you won't get the 1st ~50bases of the reference. This is known by all in space except Be…
Kevin McKernan posted the following comments about this blog post (https://twitter.com/Kevin_McKernan/status/1674047174357680131):
"Reads don't map to the margin of a reference because the given insert size you have selected in library construction won't exist. If you have 200bp inserts, you won't get the 1st ~50bases of the reference. This is known by all in space except Ben who thinks it's a discovery."
"Read mapping 101. If you make 200bp insert libraries, the likelihood of you capturing the 1st 50bp of the reference is reduced. This is just poisson sampling. https://irepertoire.com/ngs-considerations-coverage-read-length-multiplexing/"
"Secondly, there are biological reasons why those pieces of DNA aren't captured. The 5' end has a 5' cap which can't be cloned unless it's decapped. The 3' end is a variable poly A tail which won't sequence or amplify without polymerase slippage. Reduced end seq is expected."