They saying that the lung fluid is teeming with baby virus particles why don't they just purify the virus particles directly from the lung fluid and then purify the intact 30,000 nucleotide-long virus RNA and only then do the sequencing?
If they never find and purify the virus, how can anyone know if the RNA sequences of interest actually belong to "a virus"?
Maybe the RNA they studying comes from the person due to such a person being sick or just being very nervous.
Yeah. they could just purify the 'virus' directly from lung fluid and then do electrophoresis to get the intact virus RNA. Instead of all the bizarre alchemy with chicken eggs or cultures of crazy cancer cells.
I mean the entire field is based on a particle that is invisible using natural light and arguably once our bodies produce how can you tell it from other stuff our bodies produce. So it's a tough hill to climb but its monetarily advantageous to have such particles around to take the blame for stuff which I'd argue may make the 'science' easier to do...
I just checked PUBMED there's >200,000 results for "sars-cov-2" search. Yet there never was such a virus. It's just mind boggles the level of Lysenko tomfoolery.
First, we need to understand what sequencing means. Sequencing can refer to two different approaches:
1. De novo sequencing, which involves assembling a genome from scratch without a reference. This is typically used for novel organisms or when no reference genome exists.
2. Alignment-based sequencing, which maps sequencing reads to an existing reference genome to identify variations or confirm known sequences.
There are no exact numbers, but based on my research, de novo sequencing has been performed fewer than 1,000 times worldwide, while the vast majority of sequencing efforts rely on alignment.
Mathematically speaking, neither approach can prove the existence of this genome, and more reliable methods, such as overlap consensus approaches, have failed.
The samples contain a mix of human and bacterial material rather than a purified isolate consisting solely of viral material. Additionally, RACE (Rapid Amplification of cDNA Ends) was used to manually extend the sequence ends, a technique commonly employed to obtain full-length RNA sequences.
But they use the same PCR primers for the test as in the sequencing, no?
Yes, but how do we know these are specific to the said sequence - also they might have found other sequences as well..
They saying that the lung fluid is teeming with baby virus particles why don't they just purify the virus particles directly from the lung fluid and then purify the intact 30,000 nucleotide-long virus RNA and only then do the sequencing?
If they never find and purify the virus, how can anyone know if the RNA sequences of interest actually belong to "a virus"?
Maybe the RNA they studying comes from the person due to such a person being sick or just being very nervous.
This is exactly the problem!
Yeah. they could just purify the 'virus' directly from lung fluid and then do electrophoresis to get the intact virus RNA. Instead of all the bizarre alchemy with chicken eggs or cultures of crazy cancer cells.
How can so many people be fooled?
I mean the entire field is based on a particle that is invisible using natural light and arguably once our bodies produce how can you tell it from other stuff our bodies produce. So it's a tough hill to climb but its monetarily advantageous to have such particles around to take the blame for stuff which I'd argue may make the 'science' easier to do...
I just checked PUBMED there's >200,000 results for "sars-cov-2" search. Yet there never was such a virus. It's just mind boggles the level of Lysenko tomfoolery.
similar to this: https://x.com/VictorFromDE/status/1874640881992765529
I've read that Sars Cov-2 has been sequenced thousands of times. What does this really mean? It has even been SEM photographed, right?
First, we need to understand what sequencing means. Sequencing can refer to two different approaches:
1. De novo sequencing, which involves assembling a genome from scratch without a reference. This is typically used for novel organisms or when no reference genome exists.
2. Alignment-based sequencing, which maps sequencing reads to an existing reference genome to identify variations or confirm known sequences.
There are no exact numbers, but based on my research, de novo sequencing has been performed fewer than 1,000 times worldwide, while the vast majority of sequencing efforts rely on alignment.
Mathematically speaking, neither approach can prove the existence of this genome, and more reliable methods, such as overlap consensus approaches, have failed.
The samples contain a mix of human and bacterial material rather than a purified isolate consisting solely of viral material. Additionally, RACE (Rapid Amplification of cDNA Ends) was used to manually extend the sequence ends, a technique commonly employed to obtain full-length RNA sequences.